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New Tools for Behavioral and Circuit Analysis

In parallel with our efforts to understand the neurobiology of emotion systems, we are developing new tools and methodologies to enable more rapid and powerful approaches to these biological problems. The methods and enabling platform technologies we are developing include a) automated, machine vision-based quantitative measurement of behavior (in collaboration with the laboratory of Dr. Pietro Perona, Allen E. Puckett Professor of Electrical Engineering); b) new, viral-based methods for trans-neuronal circuit tracing; c) genetically based silencing of neuronal activity (in collaboration with the laboratory of Dr. Henry A. Lester, Bren Professor of Biology); and d) novel preparations for in vivo imaging of neuronal activity using genetically encoded calcium indicators (GECIs).

People

Li-Ching Lo, research specialist
Yonil Jung, graduate student
Eyrun Eyolfsdottir, graduate student
Carlos Gonzales, graduate student

Recent publications

Inagaki, H. K., Jung, Y., Hoopfer, E. D., Wong, A. M., Mishra, N., Lin, J. Y., et al. (2013). Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship. Nature Methods

Vrontou, S., Wong, A.M., Rau, K., Koerber, H.R., and Anderson, D.J. (2013) Genetic identification of C-fibers that detect massage-like stroking of hairy skin in vivo. Nature 493:669-673

Burgos-Artizzu, X. P., Dollár, P., Dayu Lin, Anderson, D. J., and Perona, P. (2012). Social behavior recognition in continuous video (pp. 1322–1329). Presented at the 2012 IEEE Conference on Computer Vision and Pattern Recognition (CVPR), IEEE. doi:10.1109/CVPR.2012.6247817

Inagaki, H. K., de-Leon, S. B.-T., Wong, A. M., Jagadish, S., Ishimoto, H., Barnea, G., et al. (2012). Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. Cell, 148(3), 583–595

Lo, L.C. and Anderson, D.J. (2011) A cre-dependent anterograde trans-neuronal virus for tracing genetically marked neural pathways. Neuron 72:938-950

Dankert, H., Wang, L., Hoopfer, E.D., Anderson, D.J., and Perona, P. (2009). Automated monitoring and analysis of social behavior in Drosophila. Nat Methods 6, 297-303. [PubMed]

Lerchner, W., Xiao, C., Nashmi, R., Slimko, E.M., van Trigt, L., Lester, H.A., and Anderson, D.J. (2007). Reversible silencing of neuronal excitability in behaving mice by a genetically targeted, ivermectin-gated Cl- channel. Neuron 54, 35-49. [PubMed]